Oup was observed. The above spectral information confirmed the structure of (two) as shown beneath. Experimental General solutions IR spectra had been recorded on a Pelkin Elmer spectrometer model 983 with KBr discs. The 1 H (400 MHz), 13C (one hundred MHz) and 2D NMR spectra had been recorded on Bruker WP200SY spectrometer. Chemical shifts, are in ppm with TMS because the internal common. Coupling constants, J have been given in Hz. MS was obtainedat 70 eV working with JOEL-MS route (Direct probe) and EI-MS were made use of for the mass spectroscopy. Plant material Gomphrena celosioides C. Mart was collected fresh in the premises of Abdulsalami Abubakar Graduate Hall, University of Ibadan, Nigeria. The identity was authenticated by Mr. Felix I. Usong of Forestry Research Institute of Nigeria, (FRIN) and Ibadan, where a specimen copy with voucher quantity FHI106429 was deposited. Extraction and isolation Air-dried coarsely powdered whole plant (1.5 Kg) was percolated with hot n-hexane (five L) for 12 h at regulated temperature of 40-50 o C. Whitish flakes have been obtained floating on the n-hexane extract soon after cooling. The white flakes had been collected and washed quite a few instances with n-hexane. The crystals were dissolved, spotted and created on TLC. TLC on n-hexane/CHCl3 showed two spots. Column chromatographic separation effected with five CHCl3 in n-hexane afforded compounds 1 (12 mg) and two (17 mg). Aurantiamide (1) White amorphous strong, mp 186-188 oC. IR (KBr) max cm-1: 3440 (OH, NH), 1639 (-CON), 722 (monosubstituted phenyl). 1 H NMR (400 MHz, CDCl3), (ppm): six.61 (1H, d, J = 7.50 Hz, H-3), six.51 (1H, d, J = 7.50 Hz, H-6), 4.03 (1H, dd, J = 7.21, six.44 Hz, H-1a), four.05 (1H, dd, J = 8.29, five.38 Hz, H-1b), four.61 (1H, m, H-2), four.19 (1H, dd, J = 4.28, four.03 Hz, H-5), 3.20 (2H, m, H-8), two.91 (2H, m, H-9), 7.20 ?7.68(15H, m, Ar-H). 13 C NMR (100 MHz, CDCl3), (ppm): 65.four(C-DOSUMU O et al. / IJPR (2014), 13 (1): 143-Figure three. Structures of aurantiamide and aurantiamide acetate.1), 50.3(C-2), 167.four(C-4), 54.5(C-5), 167.two(C-7), 37.3(C-8), 37.six(C-9), 126.8-137.two (Ar-C). EI-MS: m/z (rel. int.): 77(14) [C6H5]+, 91(17) [C7H7]+, 105(87) [C6H5CO]+, 129(5) [C6H5CH = NH2]+, 131(8) [C6H5CH = CHCO]+, 224(47) [C6H5CO-NH-CHCH2Ph]+, [C6H5CONHCH(CO)CH2C6H5]+, 252(72) 293(12) [M-(MeCO2H + C6H5CH2)]+, 353(four) [M-C6H5CH]+, 384(7) [M-H2O]+. The fragments m /z 134(4) [C6H5CH2CH = CHOH]+, and 311(eight) [M-C6H5CH2]+, are indicative from the presence in the corresponding amide.[Ir(dFppy)2(dtbbpy)]PF6 Price Aurantiamide acetate (2) colourless needle like crystal, mp 183-184 oC (uncorr.Price of 1936429-06-9 ) IR (KBr) max (cm-1): 3440 (NH), 1735 (CO2), 1639 (NHC = O) 1 H NMR (400 MHz, CDCl3), (ppm): 2.PMID:23916866 05 (3H, s, H-2″), three.04 (1H, dd, J = 8.55, 13.68, H-8), 3.18 (1H,dd, J = 5.99, 13.68, H-9), three.81 (1H,dd, J = four.27, 11.12, H-1b), three.91 (1H, dd, J = 5.13, 11.12, H-1a), 4.74 (1H, ddd, J = five.99, eight.55, = 7.68, H-2), five.96 (1H, d, J = eight.55, H-3), 6.73 (1H, d, J = 7.69, H-6), 7.05-7.69 (15H, m, Ar-H). 13 C NMR (one hundred MHz, CDCl3), (ppm): 37.5 (C-1), 49.five (C-2), 169.two (C-4), 48.4 (C-5), 170.7 (C-7), 37.3(C-8), 37.6(C-9), 174.two (C-1″), 20.7(C2″), 126.7 – 157.1 (Ar-C). EI-MS m/z (rel. int.): 76.9(27.7) [C6H5]+, 91(10.four) [C7H7]+, 105(89) [C6H5CO]+, 120(three.five) [C6H5CH = NH2]+, 131(five.5) [C6H5CH = CHCO]+, 224(17) [PhCONHCHCH2Ph]+, 252(19) [224 + CO]+.Conclusions Gomphrena celosioides contain aurantiamides and its acetate. These compounds have proved to become active against microorganism even at very low concentrations (19). This finding has supported the use of this plant in folk medicine for therapy of gastro.
