, a 2D experiment was performed, with 1 dimension getting the radiofrequency, plus the other getting the RF pulse duration. The 2D information set was then integrated over the RF pulse duration to receive the 1D ENDOR spectrum using the relative intensities in the 14N lines reflecting relative numbers of nuclei rather then relative transition probabilities. The 2D ENDOR data set (from which the 1D spectrum in Figure 5 was obtained) is shown in Figure S8 (Supporting Data).ACKNOWLEDGMENTS We’re grateful to Drs. Elizabeth Ilardi and Jonathan Loughrey for assistance using the purification of H2PD1 and Zn(HPD1)2, respectively, and to Drs. Jonathan Loughrey and Sue Roberts for assistance together with the acquisition and evaluation of X-ray diffraction information. We thank the University of Arizona and the Donors in the American Chemical Society Petroleum Investigation Fund (grant 51754-DNI3 to E.T.) for financial assistance. A.V.A. gratefully acknowledges NSF (DBI-0139459, DBI-9604939, and BIR-9224431) and NIH (S10RR020959 and S10RR026416-01) grants for the development in the EPR facility in the University of Arizona.Related CONTENTS * Supporting InformationH NMR and ENDOR spectroscopic data, X-ray crystallographic particulars, and CIF files. This material is obtainable free of charge through the online world at http://pubs.acs.org.(1) Wood, T. E.; Thompson, A. Chem. Rev. 2007, 107, 1831-1861. (2) Gryko, D. T.; Gryko, D.; Lee, C.-H. Chem. Soc. Rev. 2012, 41, 3780-3789. (three) Katayev, E. A.; Severin, K.; Scopelliti, R.; Ustynyuk, Y. A. Inorg. Chem. 2007, 46, 5465-5467. (four) Reid, S. D.; Wilson, C.; Blake, A. J.; Enjoy, J. B. Dalton Trans. 2010, 39, 418-425. (5) Halper, S. R.; Cohen, S. M. Inorg. Chem. 2005, 44, 486-488. (six) King, E. R.; Betley, T. A. Inorg. Chem. 2009, 48, 2361-2363. (7) King, E. R.; Sazama, G. T.; Betley, T. A. J. Am. Chem. Soc. 2012, 134, 17858-17861. (8) Hennessy, E. T.; Betley, T. A. Science 2013, 340, 591-595. (9) Thoi, V. S.; Stork, J. R.; Niles, E. T.; Depperman, E. C.; Tierney, D. L.; Cohen, S. M. Inorg. Chem. 2008, 47, 10533-10541. (ten) Patra, A. K.; Dube, K. S.; Sanders, B. C.; Papaefthymiou, G. C.; Conradie, J.; Ghosh, A.; Harrop, T. C. Chem. Sci. 2012, three, 364-369. (11) Sanders, B. C.; Patra, A. K.; Harrop, T. C. J. Inorg. Biochem. 2013, 118, 115-127. (12) Bro r ing, M.; Ko h ler, S.; Pietzonka, C. J. Porphyrins Phthalocyanines 2012, 16, 641-650. (13) Broring, M.; Kohler, S.; Ostapowicz, T.; Funk, M.; Pietzonka, C. Eur. J. Inorg. Chem. 2009, 3628-3635.1374829-47-6 Chemical name (14) Sessler, J.Price of 1547960-36-0 L.PMID:24211511 ; Gebauer, A.; Kral, V.; Lynch, V. Inorg. Chem. 1996, 35, 6636-6637. (15) Bennett, J. W.; Bentley, R. Adv. Appl. Microbiol. 2000, 47, 1-32. (16) Furstner, A. Angew. Chem., Int. Ed. 2003, 42, 3582-3603. (17) D’Alessio, R.; Bargiotti, A.; Carlini, O.; Colotta, F.; Ferrari, M.; Gnocchi, P.; Isetta, A.; Mongelli, N.; Motta, P.; Rossi, A.; Rossi, M.; Tibolla, M.; Vanotti, E. J. Med. Chem. 2000, 43, 2557-2565. (18) Furstner, A.; Grabowski, J.; Lehmann, C. W.; Kataoka, T.; Nagai, K. ChemBioChem. 2001, 2, 60-68. (19) Marchal, E.; Uddin, M. I.; Smithen, D. A.; Hawco, C. L. A.; Lanteigne, M.; Overy, D. P.; Kerr, R. G.; Thompson, A. RSC Adv. 2013, 3, 22967-22971. (20) Papireddy, K.; Smilkstein, M.; Kelly, J. X.; Shweta; Salem, S. M.; Alhamadsheh, M.; Haynes, S. W.; Challis, G. L.; Reynolds, K. A. J. Med. Chem. 2011, 54, 5296-5306. (21) Perez-Tomas, R.; Vinas, M. Curr. Med. Chem. 2010, 17, 2222- 2231. (22) Regourd, J.; Ali, A. A.-S.; Thompson, A. J. Med. Chem. 2007, 50, 1528-1536. (23) D z, R. I. S.; Be.