Ovel and fast method for identification of bioactive standard compounds from TCM formulas.Abbreviations TCM: standard Chinese medicine; EXD: Erxian decoction; EXD-S: mixtures of EXD person herbs decoction; EXD-C: combined decoction of EXD; SD-rat: Sprague Dawley-rat; CAT: catalase; SOD-1: superoxide dismutase 1; GPx-1: glutathione peroxidase; Ct: threshold cycle; RSD: relative regular deviation; HPLC: higher efficiency liquid chromatography; HPO: hypothalamus-pituitary-ovary. Authors’ contributions SCWS, YBZ, ZJZ and YT conceived and made the study. HPC and SWW performed the experiment and analyses. HPC, FWSC and SCWS wrote the manuscript. LXL and ZJZ offered invaluable comments. SCWS and TBN revised the manuscript. All authors read and approved the final manuscript. Author information 1 College of Chinese Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, ten Sassoon Road, Pokfulam, Hong Kong, SAR, China. 2 School of Biomedical Science, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, SAR, China.Conclusions In this study, the HPLC profiles of EXD-S and EXD-C happen to be evaluated with six identified marker chemicals, which exerted actions around the HPO axis. All six chemicals had been present at a greater level in EXD-S than in EXD-C. Four of them, including mangiferine, ferulic acid, jatrorrhizine and palmatine, have been demonstrated to become suitable regular chemical substances for high-quality manage of EXD accordingCheung et al. Chin Med (2017) 12:Web page 9 ofAcknowledgements This study was partially supported by grants in the Seed Funding Programme for Basic Research (Project Quantity 201211159146 and 201411159213), the University of Hong Kong. We thank Mr Keith Wong and Ms Cindy Lee for their technical assistances. Competing interests The authors declare that they have no competing interests. Received: 20 May possibly 2015 Accepted: 19 December9. ten. 11.12.13. References 1. Zhang LH. Research of active ingredients of Chinese classic compound medicine (CTCM) (). Prog Chem (). 1999;two(11):186. 2. Cai BC, Qin KM, Wu H, Cai H, Lu TL, Zhang XD. Chemical mechanism in the course of Chinese medicine processing (). Prog Chem (). 2012;24(four):6379. three. Deng YY, Gao WY, Chen HX, Wu SS. . Chin Tradit Herb Drugs (). 2005;36(12):19091. 4. Yi T, Lo H, Zhao Z, Yu Z, Yang Z, Chen H. Comparison of the chemical composition and pharmacological effects from the aqueous and ethanolic extracts from a Tibetan “Snow Lotus” (Saussurea laniceps) herb. Molecules. 2012;17(six):71834. 5. Sze SCW, Tong Y, Zhang YB, Zhang ZJ, Lau AS, Wong HK, Tsang KW, Ng TB. A novel mechanism: Erxian decoction, a Chinese medicine formula, for relieving menopausal syndrome.Buy55206-24-1 J Ethnopharmacol.5-Nitro-1H-pyrazole-3-carbonitrile Chemical name 2009;123(1):273.PMID:35126464 six. Hu YM, Wang YT, Sze SCW, Tsang KW, Wong HK, Liu Q, Zhong LD, Tong Y. Identification on the key chemical constituents and their metabolites in rat plasma and a variety of organs after oral administration of efficient Erxian decoction (EXD) fraction by liquid chromatography-mass spectrometry. Biomed Chromatogr. 2010;24(five):4799. 7. Okatani Y, Morioka N, Wakatsuki A, Nakano Y, Sagara Y. Function of your no cost radical-scavenger method in aromatase activity with the human ovary. Horm Res. 1993;39(Suppl 1):22. 8. Yuan ST, Du HY, Xia C. Effect of separated and combined decoction of Chinese medicinal formula on solubility of components ( ). Chin J Inf Tradit Chin Med ( ). 1999;six(7):292. 14. 15. 16. 17.18.19.20.Yi L, Xu X. Study on the precipitation reaction amongst baicalin and berberine.